Part:BBa_K3484003:Design

Intein-mediated T3(THRB) → eGFP (transcriptional unit)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 235
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 725
  Illegal NgoMIV site found at 2228
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 1640
  

Design Notes

Special considerations have been taken into account for the construction of the protein domain and can be seen at the registry of the basic part BBa_K3484000.

Source

Parts used for this composite are all from previous entries of the iGEM Registry:

• Promoter (BBa_J23100) + Ribosome Binding Site (BBa_B0034): BBa_K880005
• Protein domain (intein-mediated T3 biosensor): BBa_K3484002
• Double terminator (BBa_B0012 -BBa_B0011): BBa_B0014

References

Look for references at BBa_K3484000.